EIF4E anticorps (C-Term)

N° du produit ABIN7540529

N° du produit (Fournisseur): 200-901-mk9s

Cet anticorps Poulet Polyclonal détecte spécifiquement EIF4E dans WB, IHC et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour EIF4E anticorps (C-Term) (ABIN7540529)

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivité: Humain

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4E est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-EIF4E anticorps

Épitope: C-Term

N° du produit (Fournisseur): 200-901-mk9s

Fournisseur: Rockland

Fonction: EIF4E Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human EIF4E.

Purification: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène: Anti-EIF4E antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a near C-Terminal portion of human EIF4E conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 5 μg/mL

Commentaires:

Anti-EIF4E Antibody has been tested in Western Blot and IHC.  Expect a band at ~25-28kDa in western blot using appropriate tissues and lysates.  Positive control used: HEK293T, MOLT-4 and Daudi lysates in Western Blot, Human Lung Adenocarcinoma Tissue​ in Immunohistochemistry.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur EIF4E

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Autre désignation: EIF4E

Sujet: Chicken Anti-EIF4E Antibody, Eukaryotic Translation Initiation Factor 4E, MRNA Cap-Binding Protein, EIF-4F 25 KDa Subunit, EIF4EL1, EIF-4E, EIF4F, Eukaryotic Translation Initiation Factor 4E-Like 1, AUTS19, EIF4E1, EIF4E, CBP,EIF4E (Eukaryotic Translation Initiation Factor 4E) recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. It is a component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. EIF4E is associated with Autism 19 and Pervasive Developmental Disorder. Anti-EIF4E Antibody is useful for researcher interested in cancer research and AKT signaling.

ID gène: 1977

NCBI Accession: NP_001124150

UniProt: P06730

Pathways: BCR Signaling